간에 대한 고주파열치료 시 소나조이드 마이크로버블의 영향에 대한 토끼 간모델을 이용한 동물실험연구
- 주저자 : Min JH, Kim YS, Rhim H, Lee MW, TW Kang, KD Song, Lim HK - 영문 제목 : Effects of Sonazoid Microbubble Uptake on Radiofrequency Ablation of the Liver: Experimental Study Using a Rabbit Model - 한글 제목 : 간에 대한 고주파열치료 시 소나조이드 마이크로버블의 영향에 대한 토끼 간모델을 이용한 동물실험연구 - 저널 : Liver Int - 연도 : 2016 - 권 / 쪽 : 36 / 1187-1195 - 초록
BACKGROUND & AIMS: To investigate the differences in mechanical effects and ablation zone between radiofrequency (RF) ablation with and without Sonazoid uptake in an in vivo rabbit liver model.
METHODS: Our study was approved by the Institutional Animal Care and Use Committee. Twenty-five rabbits were randomly allotted to one of five ablation durations (i.e., 1, 2, 3, 6 and 12 min). For each rabbit, RF ablation was performed twice, before and 10 min after Sonazoid administration (i.e. control group vs. Sonazoid group), using a 1-cm internally cooled electrode (40W) equipped with a parallelly fixed pressure-monitoring device. During ablation, a 'popping' sound was perceived and recorded along with tissue pressure changes and RF ablation parameters. Then, the ablation volume and microscopic changes were compared.
RESULTS: Popping sounds were more frequently perceived in the control group (21/25 vs. 1/25, P < 0.001). The time to first pressure peak was shorter in the Sonazoid group (22.3 ± 1.1 s vs. 46.3 ± 4.4 s, P < 0.001) with similar pressures (39.8 ± 4.2 mmHg vs. 35.6 ± 4.1 mmHg, P = 0.350). Time to first roll-off and mean power output were significantly less in the Sonazoid group (17.6 ± 1.2 s vs. 71.2 ± 8.2 s, P < 0.001; 9.4 ± 0.3 W vs. 12.8 ± 0.5 W, P < 0.001). Consequently, the Sonazoid group had lower total energy and ablation volumes for all durations. Microscopically, the control group showed larger conflu-ent disruptions, whereas the Sonazoid group showed many smaller disruptions scattered throughout the ablation zones.
CONCLUSIONS: Radiofrequency ablation after Sonazoid uptake induces a smaller ablation zone than conventional RF ablation. However, it appears to ablate the liver tissue with less mechanical effects.