하이푸를 이용한 국소온열요법을 통한 전신항암제 (독소루비신)의 치료효과 상승에 대한 동물실험연구
- 주저자 : Chae SY, Kim YS, Rhim H, Park MJ, Lee JH, et al - 영문 제목 : Enhancement of therapeutic efficacy of systemically-administered doxorubicin by high-intensity focused ultrasound-induced localized hyperthermia: experimental study using SCC-7 xenograft model in mice - 한글 제목 : 하이푸를 이용한 국소온열요법을 통한 전신항암제 (독소루비신)의 치료효과 상승에 대한 동물실험연구 - 저널 : Ultrasound Med Biol - 연도 : 2014 - 권 / 쪽 : 40 / 1554-1563 - 초록 : The aim of this study was to evaluate the enhancement of the efficacy of systemic doxorubicin by pulsed high-intensity focused ultrasound (HIFU)-induced, localized mild hyperthermia. For the in vitro study, the intranuclear uptake of doxorubicin by squamous cell carcinoma (SCC)-7 cells incubated at different temperatures was compared. For the in vivo study, mice with SCC-7 tumors were assigned to either the control, conventional hyperthermia, HIFU hyperthermia, doxorubicin-alone, conventional hyperthermia + doxorubicin or HIFU hyperthermia + doxorubicin group. Conventional hyperthermia was induced by immersing the tumor in warm water (42.5°C), and HIFU hyperthermia was induced by HIFU after optimizing the parameters with direct temperature measurements (frequency = 1 MHz, pulse repetition frequency = 5 Hz, power = 12 W, duty cycle = 50%). In the in vitro study, fluorescence was more intense at 42°C than at 37°C and was time dependent. In the in vivo study, tumor growth in the HIFU hyperthermia + doxorubicin group was most prominently suppressed with the highest apoptotic index compared with all other groups (p < 0.05). Pulsed HIFU-induced localized mild hyperthermia enhanced the anti-cancer efficacy of systemic doxorubicin more than conventional mild hyperthermia.